Hotspot

trnK-UUU-C5T07_pgp085

LSC · 4163-4986 · 824 bp

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Pi 0.0543
MarkerSeek score 84.30
Primer available yes
Label trnK-UUU-C5T07_pgp085

Feature Metadata

label_name
trnK-UUU-C5T07_pgp085
feature_type
igs
region
LSC
length_bp
824
variable_sites
106
pi
0.0543
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-C5T07_pgp085_p1 GTCGTGGTCTTCCAAACCTT CTTGAAAGGGGTGCTCAACT 50.0 50.0 58.0 58.0 1006-1061 bp 1.0 91.6
trnK-UUU-C5T07_pgp085_p2 GTCGTGGTCTTCCAAACCTT TCTTGAAAGGGGTGCTCAAC 50.0 50.0 58.0 58.0 1007-1062 bp 1.0 91.6
trnK-UUU-C5T07_pgp085_p3 GTCGTGGTCTTCCAAACCTT GCAGAGACAAACGAAAAGGC 50.0 50.0 58.0 58.0 1482-1544 bp 1.0 91.4
trnK-UUU-C5T07_pgp085_p4 GTCGTGGTCTTCCAAACCTT GAAGGGGTATGTTGCTACCG 50.0 55.0 58.0 58.1 1629-1691 bp 1.0 91.3
trnK-UUU-C5T07_pgp085_p5 GTCGTGGTCTTCCAAACCTT TTGAAAGGGGTGCTCAACTC 50.0 50.0 58.0 58.0 1005-1038 bp 0.428571 62.7