Hotspot

rps2

LSC · 23453-24163 · 711 bp

Back to Galinsoga

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	CTGTGCATCTACGCCAATCT	TGAGATTCAGAAATAGTAACCGA	50.0	34.8	58.1	54.9	899 bp	1.0	40.1
rps2_p2	TGCATCTACGCCAATCTACG	TGAGATTCAGAAATAGTAACCGA	50.0	34.8	57.9	54.9	896 bp	1.0	40.1
rps2_p3	TTTTTCAAAAGGGGGAGGCA	TGAGATTCAGAAATAGTAACCGA	45.0	34.8	57.8	54.9	821 bp	1.0	40.1
rps2_p4	TTTTCAAAAGGGGGAGGCAG	TGAGATTCAGAAATAGTAACCGA	50.0	34.8	58.3	54.9	820 bp	1.0	40.1
rps2_p5	CTGTGCATCTACGCCAATCT	TTGAGATTCAGAAATAGTAACCGA	50.0	33.3	58.1	55.6	900 bp	1.0	40.1