Hotspot

psbC

LSC · 33398-34819 · 1422 bp

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Pi 0

MarkerSeek score 31.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTTCTCAAGAAATTCGCGCG	GGGAACCGAAGTGACTCCTA	45.0	55.0	57.8	58.4	1576 bp	1.0	74.6
psbC_p2	CGGCGGAAGATCCTGAATTT	GGGAACCGAAGTGACTCCTA	50.0	55.0	58.3	58.4	1558 bp	1.0	73.8
psbC_p3	AGGTATTCGTGCTTGGATGG	GGGAACCGAAGTGACTCCTA	50.0	55.0	57.7	58.4	1503 bp	1.0	73.8
psbC_p4	GGCGGAAGATCCTGAATTTG	GGGAACCGAAGTGACTCCTA	50.0	55.0	56.9	58.4	1557 bp	1.0	67.3
psbC_p5	TATTCGTGCTTGGATGGCG	GGGAACCGAAGTGACTCCTA	52.6	55.0	58.3	58.4	1500 bp	1.0	65.9