Hotspot

clpP

LSC · 69493-71507 · 2015 bp

Back to Galinsoga

Pi 0.00149

MarkerSeek score 58.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2015
variable_sites: 3
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AAACTCGGCAGGGTTCATTT	GGCTTTGTTACGTTTCCACA	45.0	45.0	58.0	56.9	2124-2131 bp	1.0	71.0
clpP_p2	AAACTCGGCAGGGTTCATTT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	58.0	58.4	2125-2132 bp	1.0	69.2
clpP_p3	AAACTCGGCAGGGTTCATTT	AGGCTTTGTTACGTTTCCAC	45.0	45.0	58.0	56.6	2125-2132 bp	1.0	68.6
clpP_p4	AACTCGGCAGGGTTCATTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.5	56.9	2123-2130 bp	1.0	67.3
clpP_p5	CGGCAGGGTTCATTTCAGAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.5	56.9	2119-2126 bp	1.0	67.3