Hotspot

psaC-ndhD

SSC · 119699-119814 · 116 bp

Back to Galinsoga

Pi 0.0259

MarkerSeek score 66.90

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 3
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	ACAGAGGATTGTGTCGGTTG	AGAAAATGAAGGAACCTGCA	50.0	40.0	57.8	55.1	297-351 bp	1.0	56.5
psaC-ndhD_p2	CAGAGGATTGTGTCGGTTGT	AGAAAATGAAGGAACCTGCA	50.0	40.0	57.8	55.1	296-350 bp	1.0	56.5
psaC-ndhD_p3	AAGAGATGTGAATCCGCCTG	AGAAAATGAAGGAACCTGCA	50.0	40.0	57.7	55.1	276-330 bp	1.0	55.3
psaC-ndhD_p4	CAGAGGATTGTGTCGGTTGT	AAGAAAATGAAGGAACCTGCA	50.0	38.1	57.8	56.0	297-351 bp	1.0	55.1
psaC-ndhD_p5	CAGAGGATTGTGTCGGTTGT	AGAAAATGAAGGAACCTGCAA	50.0	38.1	57.8	56.0	296-350 bp	1.0	55.1