Hotspot

psbC-trnS-UGA

LSC · 34820-35053 · 234 bp

Back to Galinsoga

Pi 0.0171

MarkerSeek score 62.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 4
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCTTCGTAGGGCATTTGTGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	429 bp	1.0	79.7
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	346 bp	1.0	79.5
psbC-trnS-UGA_p3	TCTTCGTAGGGCATTTGTGG	GTCTTTCGGAGAGATGGCTG	50.0	55.0	57.9	58.1	437 bp	1.0	79.3
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GTCTTTCGGAGAGATGGCTG	55.0	55.0	58.2	58.1	354 bp	1.0	79.1
psbC-trnS-UGA_p5	TCTTCGTAGGGCATTTGTGG	TCTTTCGGAGAGATGGCTGA	50.0	50.0	57.9	58.1	436 bp	1.0	78.7