Hotspot

rps11

LSC · 77881-78297 · 417 bp

Back to Galeopsis

Pi 0.0024

MarkerSeek score 41.70

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 1
pi: 0.0024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	TGGAGACAGAATAAAGCGCC	ATGTTCCGCGTTCGTTTAGA	50.0	45.0	58.0	57.9	1084-1099 bp	1.0	80.2
rps11_p2	TGGAGACAGAATAAAGCGCC	TGTTCCGCGTTCGTTTAGAT	50.0	45.0	58.0	57.9	1083-1098 bp	1.0	80.2
rps11_p3	GTGCGGGACATTCTCAGATT	ATGTTCCGCGTTCGTTTAGA	50.0	45.0	58.0	57.9	1192-1207 bp	1.0	80.1
rps11_p4	GTGCGGGACATTCTCAGATT	TGTTCCGCGTTCGTTTAGAT	50.0	45.0	58.0	57.9	1191-1206 bp	1.0	80.1
rps11_p5	CTCTTCTCATCGCAATGCCT	TGTTCCGCGTTCGTTTAGAT	50.0	45.0	58.0	57.9	1129-1144 bp	1.0	80.0