Hotspot

psaC

SSC · 115086-115331 · 246 bp

Back to Galeopsis

Pi 0

MarkerSeek score 22.30

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TGGATTCAAGTAGAGTTTTATGGA	GACCGAAAATTTATGGCGTTCA	33.3	40.9	55.5	57.9	313 bp	1.0	40.0
psaC_p2	TGGATTCAAGTAGAGTTTTATGGA	ACCGAAAATTTATGGCGTTCA	33.3	38.1	55.5	56.8	312 bp	1.0	40.0
psaC_p3	TGGATTCAAGTAGAGTTTTATGGA	AGACCGAAAATTTATGGCGT	33.3	40.0	55.5	55.7	314 bp	1.0	40.0
psaC_p4	TGGATTCAAGTAGAGTTTTATGGA	AGACCGAAAATTTATGGCGTTC	33.3	40.9	55.5	57.6	314 bp	1.0	40.0
psaC_p5	TGGATTCAAGTAGAGTTTTATGGAA	GACCGAAAATTTATGGCGTTCA	32.0	40.9	56.2	57.9	313 bp	1.0	40.0