Hotspot

petB

LSC · 73826-75218 · 1393 bp

Back to Galeopsis

Pi 0.00289

MarkerSeek score 46.80

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1393
variable_sites: 4
pi: 0.00289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAAAGTAGCTCCCGGTTG	TTCAAGCGAACCAGCCTATC	55.0	50.0	58.0	58.0	1974-1977 bp	1.0	80.9
petB_p2	GTAAAGTAGCTCCCGGTTGG	TTCAAGCGAACCAGCCTATC	55.0	50.0	58.0	58.0	1973-1976 bp	1.0	80.9
petB_p3	GGTAAAGTAGCTCCCGGTTG	CAAGCGAACCAGCCTATCTT	55.0	50.0	58.0	58.0	1972-1975 bp	1.0	80.8
petB_p4	GTAAAGTAGCTCCCGGTTGG	CAAGCGAACCAGCCTATCTT	55.0	50.0	58.0	58.0	1971-1974 bp	1.0	80.8
petB_p5	GTAAAGTAGCTCCCGGTTGG	CTTCAAGCGAACCAGCCTAT	55.0	50.0	58.0	58.0	1974-1977 bp	1.0	80.8