Hotspot

ndhG-ndhI

SSC · 116627-116991 · 365 bp

Back to Galeopsis

Pi 0.0164
MarkerSeek score 63.30
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
365
variable_sites
6
pi
0.0164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AAGACCCGATCCCAGAAAGA TCAAATTGCTTTAGGTCGGT 50.0 40.0 58.0 55.3 516-518 bp 1.0 58.7
ndhG-ndhI_p2 AAGACCCGATCCCAGAAAGA TCAAATTGCTTTAGGTCGGTT 50.0 38.1 58.0 56.1 516-518 bp 1.0 57.1
ndhG-ndhI_p3 AAGACCCGATCCCAGAAAGA ATCAAATTGCTTTAGGTCGGT 50.0 38.1 58.0 55.8 517-519 bp 1.0 55.3
ndhG-ndhI_p4 ACCCGATCCCAGAAAGACTA TCAAATTGCTTTAGGTCGGT 50.0 40.0 57.5 55.3 513-515 bp 1.0 54.7
ndhG-ndhI_p5 CCCAACGAAAAGGCCGAATA TCAAATTGCTTTAGGTCGGT 50.0 40.0 58.5 55.3 578-580 bp 1.0 54.6