Hotspot

rpl20-rps12

LSC · 68030-68800 · 771 bp

Back to Galeopsis

Pi 0.00915

MarkerSeek score 64.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 7
pi: 0.00915
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCCCGAAAACTTGATGCAA	ACAGCCCATTCGAAATGTCA	45.0	45.0	58.0	57.8	923-928 bp	1.0	79.4
rpl20-rps12_p2	TCCCCGAAAACTTGATGCAA	ACAAGACAGCCCATTCGAAA	45.0	45.0	58.0	57.7	928-933 bp	1.0	78.9
rpl20-rps12_p3	ATCCCCGAAAACTTGATGCA	ACAGCCCATTCGAAATGTCA	45.0	45.0	57.8	57.8	924-929 bp	1.0	77.8
rpl20-rps12_p4	CGGGCTATATATCCGCGTTT	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.7	57.8	877-882 bp	1.0	77.3
rpl20-rps12_p5	ATCCCCGAAAACTTGATGCA	ACAAGACAGCCCATTCGAAA	45.0	45.0	57.8	57.7	929-934 bp	1.0	77.2