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psbJ-psbL

LSC · 63724-63860 · 137 bp

Back to Galeopsis

Pi 0.0219

MarkerSeek score 65.10

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 3
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TCCTTCCAGTAGTATCGGCC	GCTATGACACAATCAAACCCG	55.0	47.6	58.3	57.6	279 bp	1.0	67.2
psbJ-psbL_p2	AGGGATGAACCTAATCCGGA	GCTATGACACAATCAAACCCG	50.0	47.6	57.5	57.6	376 bp	1.0	65.5
psbJ-psbL_p3	CAGGGATGAACCTAATCCGG	GCTATGACACAATCAAACCCG	55.0	47.6	57.4	57.6	377 bp	1.0	65.0
psbJ-psbL_p4	CCTTCCAGTAGTATCGGCCA	GCTATGACACAATCAAACCCG	55.0	47.6	58.6	57.6	278 bp	1.0	64.9
psbJ-psbL_p5	CCGATCACAAGAATACCGGT	GCTATGACACAATCAAACCCG	50.0	47.6	57.4	57.6	325 bp	1.0	64.7