Hotspot

rbcL-accD

LSC · 56473-57169 · 697 bp

Back to Galeopsis

Pi 0.012

MarkerSeek score 68.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 8
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCGCTTGTGAGGTATGGAAA	CGCGCCCTAACTCTTTCTTA	50.0	50.0	57.9	57.7	809-826 bp	1.0	80.3
rbcL-accD_p2	CCGCTTGTGAGGTATGGAAA	TTCTTTATCCCATACCCGCG	50.0	50.0	57.9	57.5	825-842 bp	1.0	78.3
rbcL-accD_p3	GCTTGCAAATGGAGTCCTGA	CGCGCCCTAACTCTTTCTTA	50.0	50.0	58.5	57.7	837-854 bp	1.0	77.4
rbcL-accD_p4	GCTTGCAAATGGAGTCCTGA	TTCTTTATCCCATACCCGCG	50.0	50.0	58.5	57.5	853-870 bp	1.0	75.4
rbcL-accD_p5	TGCAAATGGAGTCCTGAACT	CGCGCCCTAACTCTTTCTTA	45.0	50.0	57.0	57.7	834-851 bp	1.0	73.4