Hotspot

trnV-UAC-trnM-CAU

LSC · 51887-52066 · 180 bp

Back to Galeopsis

Pi 0.0278
MarkerSeek score 66.10
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
LSC
length_bp
180
variable_sites
5
pi
0.0278
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 ACCAACTGAGCTATAGCCCT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.8 58.4 244-252 bp 1.0 76.9
trnV-UAC-trnM-CAU_p2 ACCAACTGAGCTATAGCCCT TAGATGCCGGAGTCAATGGT 50.0 50.0 57.8 58.5 306-314 bp 1.0 75.8
trnV-UAC-trnM-CAU_p3 ACCAACTGAGCTATAGCCCT TTAGATGCCGGAGTCAATGG 50.0 50.0 57.8 57.4 307-315 bp 1.0 74.9
trnV-UAC-trnM-CAU_p4 ACCAACTGAGCTATAGCCCT CCGCCGTATGAAAGCAATAC 50.0 50.0 57.8 57.3 243-251 bp 1.0 74.9
trnV-UAC-trnM-CAU_p5 ACCAACTGAGCTATAGCCCT TTTGAACCAATGACTCCCGC 50.0 50.0 57.8 58.8 259-267 bp 1.0 74.0