Hotspot

petN-psbM

LSC · 29350-30125 · 776 bp

Back to Galeopsis

Pi 0.0104

MarkerSeek score 65.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 776
variable_sites: 8
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TTCCTACCGCCTTTTTGCTT	50.0	45.0	58.0	58.0	862-863 bp	1.0	81.2
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TTCCTACCGCCTTTTTGCTT	50.0	45.0	58.0	58.0	860-861 bp	1.0	81.2
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TTCCTACCGCCTTTTTGCTT	55.0	45.0	57.9	58.0	859-860 bp	1.0	80.6
petN-psbM_p4	GGGGACATAATGCACATGGA	TTCCTACCGCCTTTTTGCTT	50.0	45.0	57.6	58.0	936-937 bp	1.0	78.2
petN-psbM_p5	AGGGGACATAATGCACATGG	TTCCTACCGCCTTTTTGCTT	50.0	45.0	57.6	58.0	937-938 bp	1.0	78.2