Hotspot

rpoB-trnC-GCA

LSC · 27233-28351 · 1119 bp

Back to Galeopsis

Pi 0.011

MarkerSeek score 67.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 12
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1183-1208 bp	1.0	79.9
rpoB-trnC-GCA_p2	GTTCTTCTGTCAAACCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1259-1284 bp	1.0	79.7
rpoB-trnC-GCA_p3	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1260-1285 bp	1.0	79.6
rpoB-trnC-GCA_p4	CATTGCCTCATTTGCATCCC	TTTGGTAATCAGGCGACACC	50.0	50.0	57.8	58.2	1202-1227 bp	1.0	79.1
rpoB-trnC-GCA_p5	GTTCTTCTGTCAAACCCCGA	TTTGGTAATCAGGCGACACC	50.0	50.0	57.8	58.2	1278-1303 bp	1.0	78.8