Hotspot

rps19

Genome · 42172-42456 · 285 bp

Pi 0.0394

MarkerSeek score 63.60

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: Genome
length_bp: 285
variable_sites: 11
pi: 0.0394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGGAAAGGGTGGACAATTGG	CAATTCCCTCGACACCAGAG	50.0	55.0	58.0	58.0	1562 bp	0.5	59.8
rps19_p2	AACCCTGTAGACCATCCACA	CAATTCCCTCGACACCAGAG	50.0	55.0	58.0	58.0	1330 bp	0.5	59.6
rps19_p3	GGGTGGACAATTGGCTAGAG	CAATTCCCTCGACACCAGAG	55.0	55.0	58.0	58.0	1556 bp	0.5	59.5
rps19_p4	GCGGTAGCGAAACTTATTGC	CAATTCCCTCGACACCAGAG	50.0	55.0	57.9	58.0	1528 bp	0.5	59.2
rps19_p5	GTTGGACAAGTCGGGAATGT	CAATTCCCTCGACACCAGAG	50.0	55.0	58.1	58.0	1432 bp	0.5	59.0