Hotspot

clpP

Genome · 54975-56163 · 1189 bp

Pi 0.0823

MarkerSeek score 80.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 1189
variable_sites: 94
pi: 0.0823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AACAGGGTCAGAGGGATCAA	CTCTAACCTTCCCAACCACG	50.0	55.0	58.0	57.9	2539-2668 bp	1.0	84.4
clpP_p2	ACAGGGTCAGAGGGATCAAA	CTCTAACCTTCCCAACCACG	50.0	55.0	58.0	57.9	2538-2667 bp	1.0	84.4
clpP_p3	AACAGGGTCAGAGGGATCAA	AGCGGAACCCTAACCTAAGT	50.0	50.0	58.0	58.1	1834 bp	0.5	59.2
clpP_p4	ACAGGGTCAGAGGGATCAAA	AGCGGAACCCTAACCTAAGT	50.0	50.0	58.0	58.1	1833 bp	0.5	59.2
clpP_p5	GGTGGTGGGGGTTTACTTTT	AGCGGAACCCTAACCTAAGT	50.0	50.0	57.9	58.1	1473 bp	0.5	58.9