Hotspot

rpl14-rps8

Genome · 45740-46257 · 518 bp

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Pi 0.0699
MarkerSeek score 75.90
Primer available yes
Label rpl14-rps8

Feature Metadata

label_name
rpl14-rps8
feature_type
igs
region
Genome
length_bp
518
variable_sites
36
pi
0.0699
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rps8_p1 GTTTCATTAGCACCCGAGGT CAGCGATAGTGTCTTTACCCA 50.0 47.6 57.9 57.5 570-603 bp 1.0 72.3
rpl14-rps8_p2 GTTTCATTAGCACCCGAGGT AGCGATAGTGTCTTTACCCAC 50.0 47.6 57.9 57.5 569-602 bp 1.0 72.3
rpl14-rps8_p3 GTTTCATTAGCACCCGAGGT CGATAGTGTCTTTACCCACGA 50.0 47.6 57.9 57.2 567-600 bp 1.0 70.3
rpl14-rps8_p4 GTTTCATTAGCACCCGAGGT TCAGCGATAGTGTCTTTACCC 50.0 47.6 57.9 57.2 571-604 bp 1.0 70.1
rpl14-rps8_p5 GTTTCATTAGCACCCGAGGT GCGATAGTGTCTTTACCCACG 50.0 52.4 57.9 58.8 568-601 bp 1.0 70.0