Hotspot

rps19-rpl22

Genome · 42457-42778 · 322 bp

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Pi 0.116
MarkerSeek score 78.10
Primer available yes
Label rps19-rpl22

Feature Metadata

label_name
rps19-rpl22
feature_type
igs
region
Genome
length_bp
322
variable_sites
34
pi
0.116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl22_p1 AACCCTGTAGACCATCCACA AGTCTGAAACCAAGCGGATG 50.0 50.0 58.0 58.2 1105-1184 bp 1.0 86.3
rps19-rpl22_p2 AACCCTGTAGACCATCCACA ATCCATTCCACACGTGCAAT 50.0 45.0 58.0 58.2 1606-1685 bp 1.0 85.5
rps19-rpl22_p3 AACCCTGTAGACCATCCACA CAATTCCCTCGACACCAGAG 50.0 55.0 58.0 58.0 1330 bp 0.5 59.6
rps19-rpl22_p4 CCGCACTTGGAAGAAGAAGT CAATTCCCTCGACACCAGAG 50.0 55.0 58.1 58.0 1248 bp 0.5 58.9
rps19-rpl22_p5 AGGGAAGCGTCCTGTAGTAA CAATTCCCTCGACACCAGAG 50.0 55.0 57.8 58.0 1364 bp 0.5 58.1