Hotspot

ycf80

Genome · 72480-74258 · 1779 bp

Back to Galdieria

Pi 0.249

MarkerSeek score 78.50

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1779
variable_sites: 441
pi: 0.249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	AGTCGGCAGTGCAACATTTA	ACCACCACACCTATCGGTAA	45.0	50.0	58.1	58.1	2345 bp	0.5	58.7
ycf80_p2	AGTCGGCAGTGCAACATTTA	ACTATACCAGCTGCTAGGCA	45.0	50.0	58.1	57.9	2518 bp	0.5	58.4
ycf80_p3	GCATAAAACAGGGAGGCTGT	ACCACCACACCTATCGGTAA	50.0	50.0	58.2	58.1	2276 bp	0.5	58.2
ycf80_p4	TAAAACAGGGAGGCTGTTCG	ACCACCACACCTATCGGTAA	50.0	50.0	57.8	58.1	2273 bp	0.5	58.1
ycf80_p5	GCATAAAACAGGGAGGCTGT	ACTATACCAGCTGCTAGGCA	50.0	50.0	58.2	57.9	2449 bp	0.5	58.0