Hotspot

minD

Genome · 114269-115069 · 801 bp

Back to Galdieria

Pi 0.233

MarkerSeek score 80.80

Primer available yes

Label minD

Feature Metadata

label_name: minD
feature_type: gene
region: Genome
length_bp: 801
variable_sites: 184
pi: 0.233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
minD_p1	CTTGGACACCCTATTCCTGC	ACGTCGACCCTTAACAGTATG	55.0	47.6	58.0	57.5	2229 bp	0.5	47.7
minD_p2	TAGCTGCTTCACTTGGACAC	ACGTCGACCCTTAACAGTATG	50.0	47.6	57.8	57.5	2240 bp	0.5	46.6
minD_p3	TTCCATGGCTTTAGCTGCTT	ACGTCGACCCTTAACAGTATG	45.0	47.6	57.8	57.5	2251 bp	0.5	46.2
minD_p4	TCCATGGCTTTAGCTGCTTC	ACGTCGACCCTTAACAGTATG	50.0	47.6	58.2	57.5	2250 bp	0.5	46.1
minD_p5	GGACACCCTATTCCTGCATC	ACGTCGACCCTTAACAGTATG	55.0	47.6	57.7	57.5	2226 bp	0.5	45.9