Hotspot

ilvH

Genome · 67717-68271 · 555 bp

Back to Galdieria

Pi 0.159

MarkerSeek score 79.40

Primer available yes

Label ilvH

Feature Metadata

label_name: ilvH
feature_type: gene
region: Genome
length_bp: 555
variable_sites: 87
pi: 0.159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ilvH_p1	GTGCTACTGGTGGTTCTGTT	GGTATGGCCAAGTGGTAAGG	50.0	55.0	57.7	57.9	1066 bp	0.5	57.5
ilvH_p2	GTGCTACTGGTGGTTCTGTT	GGTAAGGCAGAGGATTGCAA	50.0	50.0	57.7	57.9	1053 bp	0.5	56.9
ilvH_p3	TACGTGCTACTGGTGGTTCT	GGTATGGCCAAGTGGTAAGG	50.0	55.0	58.4	57.9	1069 bp	0.5	56.6
ilvH_p4	GTGCTACTGGTGGTTCTGTT	AAGGGGATGTGTTGGAATGG	50.0	50.0	57.7	57.8	984 bp	0.5	56.1
ilvH_p5	GTGCTACTGGTGGTTCTGTT	AAGTCTTAAAGCGCGCAATG	50.0	45.0	57.7	57.8	1661 bp	0.5	56.0