Hotspot

chlI

Genome · 76077-77117 · 1041 bp

Back to Galdieria

Pi 0.18

MarkerSeek score 79.60

Primer available yes

Label chlI

Feature Metadata

label_name: chlI
feature_type: gene
region: Genome
length_bp: 1041
variable_sites: 186
pi: 0.18
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI_p1	GCAGATGGTCACTGGTTCAA	GCAGAAGGCAATCCAGCTAT	50.0	50.0	58.1	58.0	1739 bp	0.5	59.0
chlI_p2	GCAGATGGTCACTGGTTCAA	TTCCAGCCGTTTTAGCACTT	50.0	45.0	58.1	58.0	1326 bp	0.5	59.0
chlI_p3	GCCTTACAAGCAGATGGTCA	GCAGAAGGCAATCCAGCTAT	50.0	50.0	57.9	58.0	1748 bp	0.5	58.9
chlI_p4	TGCCTTACAAGCAGATGGTC	GCAGAAGGCAATCCAGCTAT	50.0	50.0	57.9	58.0	1749 bp	0.5	58.9
chlI_p5	GCCTTACAAGCAGATGGTCA	TTCCAGCCGTTTTAGCACTT	50.0	45.0	57.9	58.0	1335 bp	0.5	58.9