Hotspot

Gsul_orf2

Genome · 85743-86537 · 795 bp

Back to Galdieria

Pi 0.318

MarkerSeek score 79.00

Primer available yes

Label Gsul_orf2

Feature Metadata

label_name: Gsul_orf2
feature_type: gene
region: Genome
length_bp: 795
variable_sites: 252
pi: 0.318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
Gsul_orf2_p1	CAATACCATCCTGAAGGGGC	TTTTCCTGGTCCTGCTCCTA	55.0	50.0	58.0	58.0	1558 bp	0.5	59.6
Gsul_orf2_p2	CAATACCATCCTGAAGGGGC	GTGGGTCTCCGCTTTTTAGT	55.0	50.0	58.0	57.8	1802 bp	0.5	58.5
Gsul_orf2_p3	CAATACCATCCTGAAGGGGC	TTCCTGGTCCTGCTCCTATT	55.0	50.0	58.0	57.7	1556 bp	0.5	57.7
Gsul_orf2_p4	CAATACCATCCTGAAGGGGC	TTTCCTGGTCCTGCTCCTAT	55.0	50.0	58.0	57.7	1557 bp	0.5	57.7
Gsul_orf2_p5	CAGGGCCTCATGACAATCAT	TTTTCCTGGTCCTGCTCCTA	50.0	50.0	57.6	58.0	1536 bp	0.5	56.8