Hotspot

Gsul_orf1

Genome · 49304-50065 · 762 bp

Back to Galdieria

Pi 0.141

MarkerSeek score 80.30

Primer available yes

Label Gsul_orf1

Feature Metadata

label_name: Gsul_orf1
feature_type: gene
region: Genome
length_bp: 762
variable_sites: 106
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
Gsul_orf1_p1	ACAATGGGTATTGGGAGGGA	CTACCAGTTTCGCCACCTAC	50.0	55.0	58.0	58.0	1563 bp	0.5	59.9
Gsul_orf1_p2	ACAATGGGTATTGGGAGGGA	TGATGCGTCTACCAGTTTCG	50.0	50.0	58.0	58.0	1571 bp	0.5	59.9
Gsul_orf1_p3	ACAATGGGTATTGGGAGGGA	TCTACCAGTTTCGCCACCTA	50.0	50.0	58.0	58.1	1564 bp	0.5	59.4
Gsul_orf1_p4	ACAATGGGTATTGGGAGGGA	GTGTGATGCGTCTACCAGTT	50.0	50.0	58.0	57.9	1574 bp	0.5	59.3
Gsul_orf1_p5	ACAATGGGTATTGGGAGGGA	GAGTGTGATGCGTCTACCAG	50.0	55.0	58.0	58.1	1576 bp	0.5	59.3