Hotspot

ycf1

SSC · 126919-132267 · 5349 bp

Back to Galanthus

Pi 0.00903

MarkerSeek score 59.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5349
variable_sites: 88
pi: 0.00903
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTGGGAAAAACCAAGGTTCT	TGATTGGAGAATTCTTCATTTCTGT	42.9	32.0	57.3	57.0	231-261 bp	1.0	43.2
ycf1_p2	TCTGGGAAAAACCAAGGTTCT	TGGAGAATTCTTCATTTCTGT	42.9	33.3	57.3	52.7	227-257 bp	1.0	43.2
ycf1_p3	TCTGGGAAAAACCAAGGTTCT	TTGGAGAATTCTTCATTTCTGT	42.9	31.8	57.3	53.6	228-258 bp	1.0	43.2
ycf1_p4	TTCTGGGAAAAACCAAGGTTCT	TGATTGGAGAATTCTTCATTTCTGT	40.9	32.0	58.0	57.0	232-262 bp	1.0	43.1
ycf1_p5	AGCACAAATTCTGGGAAAAACC	TGATTGGAGAATTCTTCATTTCTGT	40.9	32.0	57.9	57.0	240-270 bp	1.0	43.0