Hotspot

psbB-psbT

LSC · 75769-75958 · 190 bp

Back to Galanthus

Pi 0.0115

MarkerSeek score 61.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 4
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGCGCTAGAACCTTATTCAGA	TTTTAGTCGGAACCTTGGGC	47.6	50.0	57.5	58.1	404-407 bp	1.0	68.9
psbB-psbT_p2	GGCGCTAGAACCTTATTCAG	TTTTAGTCGGAACCTTGGGC	50.0	50.0	56.0	58.1	404-407 bp	1.0	64.8
psbB-psbT_p3	TGCTCAAGTAGAATTTGGAGCA	TTTTAGTCGGAACCTTGGGC	40.9	50.0	58.0	58.1	351-354 bp	1.0	64.6
psbB-psbT_p4	TGGAGATCCAACTACAAGGAGA	TTTTAGTCGGAACCTTGGGC	45.5	50.0	57.9	58.1	318-321 bp	1.0	64.5
psbB-psbT_p5	GCGCTAGAACCTTATTCAGAGA	TTTTAGTCGGAACCTTGGGC	45.5	50.0	57.7	58.1	403-406 bp	1.0	62.1