Hotspot

petG-trnW-CCA

LSC · 68112-68225 · 114 bp

Back to Galanthus

Pi 0.0336

MarkerSeek score 68.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 7
pi: 0.0336
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GCGATCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.2	58.0	209-212 bp	1.0	81.0
petG-trnW-CCA_p2	GCGATCAGTTGGACCTTTGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.2	57.9	173-176 bp	1.0	81.0
petG-trnW-CCA_p3	GCGATCAGTTGGACCTTTGA	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.2	57.6	168-171 bp	1.0	78.8
petG-trnW-CCA_p4	GCGATCAGTTGGACCTTTGA	TCCAAAACCCGATGTCGTAG	50.0	50.0	58.2	57.6	175-178 bp	1.0	78.7
petG-trnW-CCA_p5	GCGATCAGTTGGACCTTTGA	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	58.2	57.7	207-210 bp	1.0	78.4