Hotspot

atpB-rbcL

LSC · 56371-57310 · 940 bp

Back to Galanthus

Pi 0.00919

MarkerSeek score 68.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 15
pi: 0.00919
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	GTCTCCGTTTGTGGTGACAT	45.0	50.0	57.9	58.1	1018-1047 bp	1.0	81.7
atpB-rbcL_p2	CTGGGCCAATAATTTGAGCG	GTCTCCGTTTGTGGTGACAT	50.0	50.0	57.5	58.1	1019-1048 bp	1.0	78.8
atpB-rbcL_p3	CGGGGGAAAAACAACATCCA	GTCTCCGTTTGTGGTGACAT	50.0	50.0	58.7	58.1	1042-1071 bp	1.0	77.4
atpB-rbcL_p4	CGCAGGACCAGAAGTAGTAG	GTCTCCGTTTGTGGTGACAT	55.0	50.0	57.1	58.1	964-993 bp	1.0	76.2
atpB-rbcL_p5	GGGCCAATAATTTGAGCGATG	GTCTCCGTTTGTGGTGACAT	47.6	50.0	58.0	58.1	1017-1046 bp	1.0	74.6