Hotspot

atpF-atpH

LSC · 14067-14556 · 490 bp

Back to Galanthus

Pi 0.0207

MarkerSeek score 58.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 19
pi: 0.0207
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	AGCGCTTTTATTTGCAAATCCT	50.0	36.4	57.9	57.8	588-591 bp	1.0	64.4
atpF-atpH_p2	CAGTGGCCTAAGGAAACGAA	AGCGCTTTTATTTGCAAATCCT	50.0	36.4	57.8	57.8	560-563 bp	1.0	64.0
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCATTAGCGCTTTTATTTGCA	50.0	38.1	57.9	56.5	593-596 bp	1.0	61.6
atpF-atpH_p4	CAGTGGCCTAAGGAAACGAA	GCATTAGCGCTTTTATTTGCA	50.0	38.1	57.8	56.5	565-568 bp	1.0	61.3
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GCGCTTTTATTTGCAAATCCT	50.0	38.1	57.9	56.4	587-590 bp	1.0	60.9