Hotspot

atpF

LSC · 13006-14285 · 1280 bp

Back to Gagnebina

Pi 0.00239

MarkerSeek score 61.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1280
variable_sites: 3
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTCATCTGCGCGAATGGTTA	AGTCTATCTATAAGAGGAGATGA	45.0	34.8	58.0	52.5	1388-1397 bp	1.0	41.6
atpF_p2	CGCGAATGGTTACCATGAGT	AGTCTATCTATAAGAGGAGATGA	50.0	34.8	58.3	52.5	1380-1389 bp	1.0	41.6
atpF_p3	TTCATCTGCGCGAATGGTTA	AGTCTATCTATAAGAGGAGATGAT	45.0	33.3	58.0	53.1	1388-1397 bp	1.0	41.6
atpF_p4	AATTTCATCTGCGCGAATGG	AGTCTATCTATAAGAGGAGATGA	45.0	34.8	57.5	52.5	1391-1400 bp	1.0	41.5
atpF_p5	ATTTCATCTGCGCGAATGGT	AGTCTATCTATAAGAGGAGATGA	45.0	34.8	58.6	52.5	1390-1399 bp	1.0	41.5