Hotspot

psbE-petL

LSC · 68954-70334 · 1381 bp

Back to Gagnebina

Pi 0.00295

MarkerSeek score 62.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1381
variable_sites: 4
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.0	58.2	1563-1584 bp	1.0	79.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.9	58.2	1552-1573 bp	1.0	79.6
psbE-petL_p3	TGACAAATAACCAACCCGCA	TCGTATCTTGCTCAGACCGA	45.0	50.0	58.0	58.3	1562-1583 bp	1.0	79.2
psbE-petL_p4	CAACCCGCAATGAATAGGGA	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.9	58.3	1551-1572 bp	1.0	78.9
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1475-1496 bp	1.0	78.8