Hotspot

accD-psaI

LSC · 63440-64130 · 691 bp

Back to Gagnebina

Pi 0.00945

MarkerSeek score 68.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 6
pi: 0.00945
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCATGCTTTCTTTCCTT	CCATTGCAAATGCCGGAAAT	45.0	45.0	56.3	58.0	749-798 bp	1.0	70.8
accD-psaI_p2	GCTCCATGCTTTCTTTCCTT	AAGAAGCCATTGCAAATGCC	45.0	45.0	56.3	57.9	755-804 bp	1.0	70.2
accD-psaI_p3	GCTCCATGCTTTCTTTCCTTT	CCATTGCAAATGCCGGAAAT	42.9	45.0	57.1	58.0	749-798 bp	1.0	68.8
accD-psaI_p4	GCTCCATGCTTTCTTTCCTTT	AAGAAGCCATTGCAAATGCC	42.9	45.0	57.1	57.9	755-804 bp	1.0	68.2
accD-psaI_p5	GCTCCATGCTTTCTTTCCTT	GCCGGAAATACTAGGCCTAC	45.0	55.0	56.3	57.5	738-787 bp	1.0	67.4