Hotspot

rbcL-accD

LSC · 61001-61906 · 906 bp

Back to Gagnebina

Pi 0.00346

MarkerSeek score 62.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 3
pi: 0.00346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CGTCACTGCCACTGGTATTT	55.0	50.0	58.0	58.2	1082-1111 bp	1.0	81.3
rbcL-accD_p2	TTCGTGAGGCTAGCAAATGG	CGTCACTGCCACTGGTATTT	50.0	50.0	58.3	58.2	1087-1116 bp	1.0	79.5
rbcL-accD_p3	GGCTAGCAAATGGAGTCCTG	CGTCACTGCCACTGGTATTT	55.0	50.0	58.3	58.2	1080-1109 bp	1.0	79.0
rbcL-accD_p4	GTGAGGCTAGCAAATGGAGT	CGTCACTGCCACTGGTATTT	50.0	50.0	57.6	58.2	1084-1113 bp	1.0	78.3
rbcL-accD_p5	AGGCTAGCAAATGGAGTCCT	CGTCACTGCCACTGGTATTT	50.0	50.0	58.4	58.2	1081-1110 bp	1.0	78.3