Hotspot

trnM-CAU-atpE

LSC · 56570-56844 · 275 bp

Back to Gagnebina

Pi 0.0218

MarkerSeek score 52.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 275
variable_sites: 6
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	ACGAGTAGAGGCTATCAATA	50.0	40.0	58.4	52.4	355-356 bp	1.0	41.4
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	ACGAGTAGAGGCTATCAATAT	50.0	38.1	58.4	53.1	355-356 bp	1.0	41.4
trnM-CAU-atpE_p3	GCGGGAGTCATTGGTTCAAA	ACGAGTAGAGGCTATCAATA	50.0	40.0	58.8	52.4	340-341 bp	1.0	41.4
trnM-CAU-atpE_p4	GTATTGCTTTCATACGGCGG	ACGAGTAGAGGCTATCAATA	50.0	40.0	57.3	52.4	356-357 bp	1.0	41.3
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	ACGAGTAGAGGCTATCAATAT	50.0	38.1	57.3	53.1	356-357 bp	1.0	41.3