Hotspot

psaA-ycf3

LSC · 46031-46814 · 784 bp

Back to Gagnebina

Pi 0

MarkerSeek score 63.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	893-930 bp	1.0	83.2
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	899-936 bp	1.0	83.0
psaA-ycf3_p3	AGAAATGACCGGGTTTAGCC	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	911-948 bp	1.0	83.0
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	924-961 bp	1.0	82.9
psaA-ycf3_p5	AGAAATGACCGGGTTTAGCC	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	917-954 bp	1.0	82.8