Hotspot

trnY-GUA-trnE-UUC

LSC · 34214-34279 · 66 bp

Back to Gagnebina

Pi 0.0152
MarkerSeek score 61.20
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
66
variable_sites
1
pi
0.0152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 TTTCATGGTGGATCGGCAAA AAGTATGCCCCCATCGTCTA 45.0 50.0 58.1 57.9 253 bp 1.0 78.9
trnY-GUA-trnE-UUC_p2 ATTTCATGGTGGATCGGCAA AAGTATGCCCCCATCGTCTA 45.0 50.0 57.9 57.9 254 bp 1.0 78.4
trnY-GUA-trnE-UUC_p3 TCATGGTGGATCGGCAAATT AAGTATGCCCCCATCGTCTA 45.0 50.0 57.9 57.9 251 bp 1.0 78.4
trnY-GUA-trnE-UUC_p4 TTCATGGTGGATCGGCAAAT AAGTATGCCCCCATCGTCTA 45.0 50.0 57.9 57.9 252 bp 1.0 78.4
trnY-GUA-trnE-UUC_p5 CCAGCGTAGACATATTGCCA AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 209 bp 1.0 77.7