Hotspot

rpoB-trnC-GCA

LSC · 29115-30330 · 1216 bp

Back to Gagnebina

Pi 0.00332

MarkerSeek score 53.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1216
variable_sites: 4
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CACATTTACACCCCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1289-1299 bp	1.0	77.3
rpoB-trnC-GCA_p2	CCCACATTTACACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.4	58.1	1291-1301 bp	1.0	76.7
rpoB-trnC-GCA_p3	ATTCCCACATTTACACCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1294-1304 bp	1.0	75.9
rpoB-trnC-GCA_p4	ACATTTACACCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.4	58.1	1288-1298 bp	1.0	75.3
rpoB-trnC-GCA_p5	CACATTTACACCCCCAAGCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1271-1281 bp	1.0	75.1