Hotspot

trnK-UUU-rps16

LSC · 4407-5461 · 1055 bp

Back to Gagnebina

Pi 0.00676
MarkerSeek score 54.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1055
variable_sites
4
pi
0.00676
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.56
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 840-1262 bp 1.0 83.5
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC CCTTGAAAAAGGCGCTCAAC 55.0 50.0 57.9 58.2 845-1267 bp 1.0 81.8
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC CTTGAAAAAGGCGCTCAACC 55.0 50.0 57.9 58.2 844-1266 bp 1.0 81.8
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TGAAAAAGGCGCTCAACCTA 55.0 45.0 57.9 57.7 842-1264 bp 1.0 81.5
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC AGGCGGGTGTTTTTATGGAA 55.0 45.0 57.9 57.7 795-1217 bp 1.0 81.3