Hotspot

ycf1

SSC · 119525-124780 · 5256 bp

Back to Gagea

Pi 0.0248
MarkerSeek score 56.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5256
variable_sites
249
pi
0.0248
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CCATTCATTCATTCCGGCCT CAAGGAATGGGAGCGAGAAA 50.0 50.0 58.3 57.9 1153-1165 bp 1.0 82.3
ycf1_p2 CCATTCATTCATTCCGGCCT TTTCAAGGAATGGGAGCGAG 50.0 50.0 58.3 57.9 1156-1168 bp 1.0 82.2
ycf1_p3 TCCATTCATTCATTCCGGCC CAAGGAATGGGAGCGAGAAA 50.0 50.0 58.3 57.9 1154-1166 bp 1.0 82.2
ycf1_p4 TCCATTCATTCATTCCGGCC TTTCAAGGAATGGGAGCGAG 50.0 50.0 58.3 57.9 1157-1169 bp 1.0 82.2
ycf1_p5 CCATTCATTCATTCCGGCCT AGGAATGGGAGCGAGAAATG 50.0 50.0 58.3 57.7 1151-1163 bp 1.0 80.5