Hotspot

ndhG-ndhI

SSC · 114464-114808 · 345 bp

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Pi 0.0512
MarkerSeek score 80.60
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
345
variable_sites
33
pi
0.0512
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TGTATTGATCCAGGTAAATCCA ACAAGGGTAAATACCTCGATTCA 36.4 39.1 54.6 57.3 396-426 bp 1.0 50.4
ndhG-ndhI_p2 TGTATTGATCCAGGTAAATCCA AGACAAGGGTAAATACCTCGA 36.4 42.9 54.6 55.8 398-428 bp 1.0 50.3
ndhG-ndhI_p3 ATGTATTGATCCAGGTAAATCCA AGACAAGGGTAAATACCTCGA 34.8 42.9 55.1 55.8 399-429 bp 1.0 50.3
ndhG-ndhI_p4 TGTATTGATCCAGGTAAATCCAT AGACAAGGGTAAATACCTCGA 34.8 42.9 55.1 55.8 398-428 bp 1.0 50.3
ndhG-ndhI_p5 TGTATTGATCCAGGTAAATCCA AGACAAGGGTAAATACCTCGAT 36.4 40.9 54.6 56.3 398-428 bp 1.0 50.3