Hotspot

psaC-ndhE

SSC · 113027-113445 · 419 bp

Pi 0.0534

MarkerSeek score 78.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 419
variable_sites: 38
pi: 0.0534
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTTTGGACCCTTGGAGATCG	50.0	55.0	58.1	58.0	1250-1281 bp	1.0	84.9
psaC-ndhE_p2	GAACCCGCGAATATAGGCAA	CTTTGGACCCTTGGAGATCG	50.0	55.0	58.1	58.0	1579-1610 bp	1.0	83.9
psaC-ndhE_p3	CTGGCCAAGCTGCTAAAGTA	CTTTGGACCCTTGGAGATCG	50.0	55.0	57.9	58.0	1842-1873 bp	1.0	83.8
psaC-ndhE_p4	TGGCCAAGCTGCTAAAGTAG	CTTTGGACCCTTGGAGATCG	50.0	55.0	57.9	58.0	1841-1872 bp	1.0	83.8
psaC-ndhE_p5	AAATAGGTCCCACGGAAAGC	CTTTGGACCCTTGGAGATCG	50.0	55.0	57.9	58.0	1794-1825 bp	0.75	73.1