Hotspot

ndhF-rpl32

SSC · 108502-109028 · 527 bp

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Pi 0.0737
MarkerSeek score 81.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
527
variable_sites
63
pi
0.0737
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCGTATTGATATGTTTGTTCCA GAAAGAGCTTTTACTGCCGT 34.8 45.0 56.9 56.4 630-669 bp 1.0 51.9
ndhF-rpl32_p2 TGCGTATTGATATGTTTGTTCCA AGAAAGAGCTTTTACTGCCGT 34.8 42.9 56.9 57.9 631-670 bp 1.0 51.8
ndhF-rpl32_p3 TCCATGCGTATTGATATGTTTGT AGAAAGAGCTTTTACTGCCGT 34.8 42.9 56.7 57.9 635-674 bp 1.0 51.8
ndhF-rpl32_p4 ATGCGTATTGATATGTTTGTTCCA AGAAAGAGCTTTTACTGCCGT 33.3 42.9 57.3 57.9 632-671 bp 1.0 51.8
ndhF-rpl32_p5 TGCGTATTGATATGTTTGTTCCAT AGAAAGAGCTTTTACTGCCGT 33.3 42.9 57.3 57.9 650-670 bp 0.75 40.4