Hotspot

psaJ-rpl33

LSC · 63692-64231 · 540 bp

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Pi 0.064
MarkerSeek score 85.60
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
540
variable_sites
64
pi
0.064
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ATCGTTTATTCCCAGACGCC ACATCTTTACCCTTGGCCAT 50.0 45.0 58.1 56.8 601-699 bp 1.0 79.9
psaJ-rpl33_p2 TTGGGTCTTTAGCGGGTCTA ACATCTTTACCCTTGGCCAT 50.0 45.0 58.1 56.8 634-732 bp 1.0 79.3
psaJ-rpl33_p3 TGGGTCTTTAGCGGGTCTAT ACATCTTTACCCTTGGCCAT 50.0 45.0 57.8 56.8 633-731 bp 1.0 78.5
psaJ-rpl33_p4 ATGGTTTGGGTCTTTAGCGG ACATCTTTACCCTTGGCCAT 50.0 45.0 58.2 56.8 639-737 bp 1.0 78.4
psaJ-rpl33_p5 CGTTTATTCCCAGACGCCTT ACATCTTTACCCTTGGCCAT 50.0 45.0 58.3 56.8 599-697 bp 1.0 78.2