Hotspot

trnW_CCA-trnP_UGG

LSC · 63038-63115 · 78 bp

Back to Gagea

Pi 0.0641
MarkerSeek score 78.70
Primer available yes
Label trnW_CCA-trnP_UGG

Feature Metadata

label_name
trnW_CCA-trnP_UGG
feature_type
igs
region
LSC
length_bp
78
variable_sites
10
pi
0.0641
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW_CCA-trnP_UGG_p1 GCGTTCTACCGAACTGAACT CACGGGTTCAAATCCTGTCA 50.0 50.0 57.9 58.1 132-135 bp 1.0 84.6
trnW_CCA-trnP_UGG_p2 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 134-137 bp 1.0 84.5
trnW_CCA-trnP_UGG_p3 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 50.0 50.0 57.9 58.1 135-138 bp 1.0 84.5
trnW_CCA-trnP_UGG_p4 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 145-148 bp 1.0 84.1
trnW_CCA-trnP_UGG_p5 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 146-149 bp 1.0 84.1