Hotspot

trnT_UGU-trnL_UAA

LSC · 44057-44836 · 780 bp

Back to Gagea

Pi 0.0543
MarkerSeek score 88.50
Primer available yes
Label trnT_UGU-trnL_UAA

Feature Metadata

label_name
trnT_UGU-trnL_UAA
feature_type
igs
region
LSC
length_bp
780
variable_sites
72
pi
0.0543
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
88.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT_UGU-trnL_UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 716-897 bp 1.0 87.9
trnT_UGU-trnL_UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 748-929 bp 1.0 86.8
trnT_UGU-trnL_UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 710-891 bp 1.0 86.7
trnT_UGU-trnL_UAA_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 742-923 bp 1.0 85.6
trnT_UGU-trnL_UAA_p5 CTAACCTCTGAGCTAAGCGG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.5 58.2 708-889 bp 1.0 85.1