Hotspot

psbI-trnS_GCU

LSC · 7425-7565 · 141 bp

Back to Gagea

Pi 0.052
MarkerSeek score 79.90
Primer available yes
Label psbI-trnS_GCU

Feature Metadata

label_name
psbI-trnS_GCU
feature_type
igs
region
LSC
length_bp
141
variable_sites
14
pi
0.052
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS_GCU_p1 GCAATCCTGGACGTGAAGAA GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.2 58.2 176-187 bp 1.0 86.8
psbI-trnS_GCU_p2 GCAATCCTGGACGTGAAGAA TTCGAATCCCTCTCTCTCCG 50.0 55.0 58.2 58.3 173-184 bp 1.0 86.1
psbI-trnS_GCU_p3 GCAATCCTGGACGTGAAGAA GGTTCGAATCCCTCTCTCTC 50.0 55.0 58.2 56.8 175-186 bp 1.0 79.3
psbI-trnS_GCU_p4 GCAATCCTGGACGTGAAGAA GTTCGAATCCCTCTCTCTCC 50.0 55.0 58.2 56.8 174-185 bp 1.0 79.3
psbI-trnS_GCU_p5 GCAATCCTGGACGTGAAGAA TCGAATCCCTCTCTCTCCG 50.0 57.9 58.2 57.6 172-183 bp 1.0 77.5