Hotspot

tsf

LSC · 39108-39710 · 603 bp

Back to Fucus

Pi 0.00332
MarkerSeek score 49.70
Primer available yes
Label tsf

Feature Metadata

label_name
tsf
feature_type
gene
region
LSC
length_bp
603
variable_sites
2
pi
0.00332
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tsf_p1 ACCGTTGGTTAGGAGGTACA ATATCATTGGTTGGGGCTGC 50.0 50.0 58.0 58.3 1628 bp 1.0 77.8
tsf_p2 ACCGTTGGTTAGGAGGTACA GCTGCAAATTCACCTTCAGG 50.0 50.0 58.0 57.6 1613 bp 1.0 77.3
tsf_p3 ACCGTTGGTTAGGAGGTACA GGCTGCAAATTCACCTTCAG 50.0 50.0 58.0 57.6 1614 bp 1.0 77.3
tsf_p4 ACCGTTGGTTAGGAGGTACA CACCTGCCCAATTACAACCA 50.0 50.0 58.0 58.4 1564 bp 1.0 77.2
tsf_p5 ACCGTTGGTTAGGAGGTACA ACCTGCCCAATTACAACCAA 50.0 45.0 58.0 57.6 1563 bp 1.0 77.1