Hotspot

trnP(tgg)

LSC · 27786-27859 · 74 bp

Back to Fucus

Pi 0.027
MarkerSeek score 68.00
Primer available yes
Label trnP(tgg)

Feature Metadata

label_name
trnP(tgg)
feature_type
tRNA
region
LSC
length_bp
74
variable_sites
2
pi
0.027
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP(tgg)_p1 ATGCCGCTAAAGAATCAGCA CAAACTGCTCTACTCCGCAT 45.0 50.0 58.0 58.0 267 bp 1.0 80.1
trnP(tgg)_p2 TGCCGCTAAAGAATCAGCAT CAAACTGCTCTACTCCGCAT 45.0 50.0 58.0 58.0 266 bp 1.0 80.1
trnP(tgg)_p3 ATGCCGCTAAAGAATCAGCA AACGAGCTACCAAACTGCTC 45.0 50.0 58.0 58.2 277 bp 1.0 78.6
trnP(tgg)_p4 TGCCGCTAAAGAATCAGCAT AACGAGCTACCAAACTGCTC 45.0 50.0 58.0 58.2 276 bp 1.0 78.6
trnP(tgg)_p5 TGCCGCTAAAGAATCAGCAT CAACGAGCTACCAAACTGCT 45.0 50.0 58.0 58.5 277 bp 1.0 76.3